Figure 2.

Yeast transactivation assay. The full-length and various truncated fragments of PALM1 were cloned into the pGBKT7 DNA-BD vector (Clonetech). The resulting plasmids were transformed into the yeast (Saccharomyces cerevisiae) Y2HGold strain (Clonetech). (A) pGBKT7 DNA-BD PALM1 fusion constructs. (1) BD, empty vector; (2) BD-PALM1FL, BD-full-length PALM1 fusion; (3) BDPALM1CΔ1, BD-PALM1 C-terminal deletion 1 fusion; (4) BD-PALM1CΔ2, BD-PALM1 C-terminal deletion 2 fusion; (5) BD-PALM1NΔ, BD-PALM1 N-terminal deletion fusion. (B) Yeast cultures at O.D.₆₀₀ = 0.5 were diluted as specified and grown at 30°C on YPD medium for 48 hrs. (C) Yeast cultures at O.D.₆₀₀ = 0.5 were diluted as specified and grown at 30°C on SD (synthetically defined) medium without tryptophan, histidine and adenine for 48 hrs. Table 1 lists primers used.