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. 2010 Dec 1;5(12):1607–1612. doi: 10.4161/psb.5.12.13941

Table 2.

Comparison of kinetic parameters of AtGH3-12, OsGH3-8, and AtGH3-17

	Assay method	Kinetic parameter	Assay method	Kinetic parameter
AtGH3-12	HPLC^a	V_max = 20.4 min⁻¹	coupled UV/Vis^b	V_max = 13.2 min⁻¹
	HPLC^a	K_m^4-HBA = 450 µM	coupled UV/Vis^b	K_m^4-HBA = 63 µM
	adenylation^a	K_m^ATP = 791 µM	coupled UV/Vis^b	K_m^ATP = 99 µM
	none	not determined	coupled UV/Vis^b	K_m^Glu = 3.83 mM
OsGH3-8	mass spec^c	V_max = 21.1 min⁻¹	coupled UV/Vis^d	V_max = 20.6 min⁻¹
	mass spec^c	K_m^IAA = 123 µM	coupled UV/Vis^d	K_m^IAA = 187 µM
	mass spec^c	K_m^ATP = 50 µM	coupled UV/Vis^d	K_m^ATP = 36 µM
	mass spec^c	K_m^Asp = 1.58 mM	coupled UV/Vis^d	K_m^Asp = 3.91 mM
AtGH3-17			coupled UV/Vis^b	V_max = 7.4 min⁻¹
			coupled UV/Vis^b	K_m^IAA = 58 µM
			coupled UV/Vis^b	K_m^ATP = 138 µM
			coupled UV/Vis^b	K_m^Glu = 0.54 mM

^a

Data summarized from Okrent, et al. 2009.⁴⁵

^b

Protein was expressed, purified, and assayed, as described in Chen, et al. 2010;⁴⁰ with errors of less than 15% for n = 3.

^c

Data summarized from Chen, et al., 2009.³⁹

^d

Data summarized from Chen, et al. 2010.⁴⁰