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. 2010 Aug 1;5(8):959–967. doi: 10.4161/psb.5.8.11768

Figure 2.

Figure 2

Neutrons affect the expression of different auxin and senescence related genes in Arabidopsis plants. To study the effect of neutron radiation, 35-d-old plants, were irradiated with different doses of neutrons (30, 50 and 76 mGy). The expression profile of auxin response factors (ARF1, 3, 19), and auxin activated genes (AUX/IAA3, 6, 7), are shown in wild-type Col plants (A and B), together with that of two Arabidopsis auxin transport mutants: aux1 (C and D), and eir1 (E and F). Note = this assay was performed by a quantitative real time qPCR analysis 1 hour after the end of neutron irradiation at three different doses: 30, 50 and 76 mGy, and after 24 h of recovery (R24, gray bars). Only results obtained at 50 mGy (gray bars) neutron dose are shown. Relative amounts were normalized with respect to ACTIN8 expression level as log2 of the relative mRNA level, calibrated to the not-treated control plants (= 0). Log2 < 0 represents a downregulation, and log2 > 0 represents an upregulation of gene expression. Bars represent the means SD (n = 4–7).