Figure 2.

Effects of OsCIPK14/15 suppression on MAMP-induced ROS generation. Time course of ROS (·O₂⁻) generation in the cell suspension after TvX/EI X treatment (120 µg mL⁻¹) (A) and N-acethylchitoheptaose (10 µM) (B). Cells of five days after subculture were washed and resuspended in fresh growth medium 30 min before measurement. A 250 µl aliquot of cells was collected at the indicated time and treated with 2 µM MC LA (Molecular Probes, Eugene, OR). The ·O₂⁻-dependent chemiluminescence was measured with a Lumicounter 2500 (Microtech Nition, Chiba, Japan) with continuous aeration by shaking of the vial. Average values and standard errors of three independent experiments for the control line (black diamond) and two independent RNAi lines (white square for RNAi-1 and white triangle for RNAi-2) are shown.