Effect of SNP on survival rates of Arabidopsis seedlings. (A) Six-day-old wild-type seedlings grown at 22°C were pretreated with 1 mL of a different concentrations (1, 0 mM; 2, 10 mM; 3, 20 mM; 4, 50 mM; or 5, 100 mM) of SNP as an NO donor. After 24 h, they were exposed to 45°C for 60 min, then returned to 22°C and photographed 6 d later. (B) Survival ratios of the seedlings. The data are the mean ± SE of at least four independent experiments, with 50 seedlings per experiment.