Table 1.
Summary of changes in expression, interaction, and localization of SNAREs by the dark
| Annotation | SYP73 | SYP72 | VTI14 | VAMP711 | SYP23 | SYP51 | VAMP712 |
| Gene locus | AT3G61450 | AT3G45280 | AT5G39630 | AT4G32150 | AT4G17730 | AT1G16240 | AT2G25340 |
| Default localization# | ER/PM | ER/PM | Endosome | Vacuole | LE/Vacuole | Endosome | TGN/Vacuole |
| Expression* (fold change) | 7.69 | 5.56 | 5.00 | 3.45 | 3.33 | 3.12 | 2.63 |
| Interaction** (fold change) | - | - | - | - | - | - | - |
| Localization in the root tip*** | - | - | - | - | - | - | - |
| Annotation | SYP124 | SYP122 | SYP24 | MEMB11 | SYP111 | SYP121 | SYP132 |
| Gene locus | AT1G61290 | AT3G52400 | AT1G32270 | AT2G36900 | AT1G08560 | AT3G11820 | AT5G08080 |
| Default localization# | PM | PM | LE/Vacuole | ER/TGN | DP | PM | PM |
| Expression* (fold change) | 2.56 | 2.50 | 2.27 | 2.12 | 0.38 | 0.86 | 0.97 |
| Interaction** (fold change) | - | 4.46 | - | - | - | 0.28 | 0.73 |
| Localization in the root tip*** | - | PM | - | - | Vacuole | Vacuole | PM |
| Control |
Localizations defined by Bassham, et al. (2008) and Uemura, et al. (2004). ER, endoplasmic reticulum; PM, plasma membrane; LE, late endosome; TGN, trans Golgi network; DP, division plane.
Results of the analysis on microarray data (Dohmann, et al. 2008) by Genevestigator V3 with a default setting (Hruz, et al. 2008). Expression signals in Arabidopsis seedlings germinated in the dark for 7 days are divided by that in seedlings germinated in the light for 7 days. SNAREs that show higher than 2 fold or lower than 0.5 fold are listed. SYP121 and SYP132 are also listed for the comparison.
Results of the split luciferase complementation assays. RLU (relative luminescence unit) in Arabidopsis protoplasts incubated in the dark for 16 h is divided by that in Arabidopsis protoplasts incubated in the light for 16 h. The interaction between SYP122 and VAMP721 or between SYP121 and VAMP722 or between SYP132 and VAMP722 is measured. The data of SYP121 and SYP132 are from the previous publication (Kato, et al. 2010).
Results of the GFP observation in the dark. Transgenic Arabidopsis that expresses GFP-SYP122, GFP-SYP121 or GFP-SYP111 (by the promoter of syp122, syp121 or syp111 respectively) is observed with a confocal microscope. The seedlings are grown in the dark (24 h) or in the light (16 h light 8 h dark) for 5 days before the observation. Subcellular localizations in the root tip are shown. GFP-SYP121 and GFP-SYP111 are localized in the plasma membrane and division planes, respectively, in the root tip when the seedlings are grown in the light. GFP-SYP122 is not detectable in the root tip when the seedlings are grown in the light. The data of SYP121 and SYP132 are from the previous publication (Kato, et al. 2010). -, not analyzed.