Table 2.

SSRs used to detect polymorphisms in Jatropha species.

SSR locus	Repeat motif	Primer sequence	Location of repeat motif	No. of alleles detected	HO	HE	PIC
JM8	(TC)23	F: GAATTTAGAAGCCACATTTGAGACG R: CCTATGTAACCCAAGAAAGACGATG	Intron	3	0.7654	0.7654	0.7
JM10	(GAA)15	F: TGGAAGACGAATACTATGACGATGA R: CAGGTGCTACTTCTTCTTCTTCAGG	Exon	2	0.5925	0.5926	0.5
JM11	(CAT)12	F: GCATGCAAACCCTGAATTATGTACT R: GCTGCTGACTCTGTTTCTCCTTCTA	Exon	2	0.5679	0.5679	0.5
JM15	(TA)42	F: TGTAGATAGCCTTAGCTGTGCATTG R: GTACTCTCGAGGGAGTTGATTGTGT	Non genic	2	0.4938	0.4938	0.4
JM16	(TA)38	F: TTGGCTCATAATAACTCCTCAAAGC R: GCGAGTGCTGTCTAAAGCCTAATTT	Non genic	2	0.6913	0.6914	0.6
JM17	(TA)31	F: CTTCTCAGCAACATCACATCAAACT R: CGCTAAGTTACATAGGACAAAGGGA	5′UTR	3	0.6172	0.6173	0.6
JM18	(AT)25	F: ATTCAGGCCATCCACATAGTCTAAC R: GACCCTATTGATTGATTTAAGAGCC	5′UTR	2	0.5925	0.5926	0.5
JM19	(TA)33	F: GCCTTAGTTGTGCATTGCTCTATTT R: ACTCAAACTTATGTCCCAATCGTCT	5′UTR	2	0.7407	0.7407	0.7
JM20	(TA)23	F: AGATTTAGAAATGGTAATAGGGCGG R: GACCTATCCGTGTCGTGTAGATTT	5′UTR	7	0.6419	0.642	0.6
JM21	(AT)25	F: GCAAGAAATAAGGTACAACCGAAAC R: GTGAGCAATTACCAAAGGAAACAAG	5′UTR	2	0.6913	0.6914	0.6
JM22	(AT)29	F: ATGCTATCGGAATAGATCCTTCGAG R: TGGTAAACAAGAGTTGAGGGTTAGG	5′UTR	3	0.4444	0.4444	0.3
JM23	(AT)32	F: GAGATGGAAATGATTGGTGTTGAGT R: CGCCTCATCCTCACATTATACACTT	5′UTR	3	0.7654	0.7654	0.7
JM24	(AT)20	F: TGATGGATTGAGAACTGAAGAGGAT R: ACTCTAATTAGGCCCAGATTCCAAC	5′UTR	4	0.6913	0.6914	0.6
JM25	(CT)20	F: CTGACATATCTTATTGGGTGTGGAA R: TGTAAGAGTATCATCCATTTGCCAG	5′UTR	2	0.6913	0.6914	0.6
JM26	(TC)18	F: GCCTTTAAGAGATTGATGGCAACTA R: AAGTATTCATATGCCCTAAGCCTCC	3′UTR	2	0.7160	0.716	0.7
JM27	(TA)24	F: TTGGAGGTTACAATCAATGGCA R: GCATGTGCCCGAATTGAATA	3′UTR	3	0.7654	0.7654	0.7
JM28	(AT)27	F: CCATTTGGTGTTAATCACATGAGTC R: GACAATAGTGATGTTGGATTCCACT	3′UTR	2	0.7160	0.716	0.7
JM29	(AT)25	F: CTGTTTGAGGATCAGACTTTGAAGC R:AAAGAAATAATGAGGAGGGAGGTTG	Intron	2	0.7160	0.716	0.7
JM30	(AT)25	F: GCATGGAAATTCAATTCTGTGCTAC R: TTTGGTGATGAGGATTGTTGCT	Intron	2	0.7160	0.716	0.7
JM31	(ATA)19	F: TGCTCCCAGTAAGCATAAGAAGAAG R: CTTGCTCGGTTACCATTACCATTAC	Intron	2	0.7160	0.716	0.7
JM32	(AT)22	F: AGCAAGAAACCATACTTCGAGTGTC R: GAGATGCCAACCTTTGTGATTAGTT	Intron	2	0.6666	0.6667	0.6
JM33	(GA)21	F: TTCAATAACAGATTTGGCTAGGCTC R: GACAATTGAAAGGTGCAATCCTAGT	Intron	3	0.4938	0.4938	0.4
JM34	(AT)24	F: CAGACCCATCTGATCATCATTGTAG R: TCCTCAGGTAAATTGCTCATCTTTC	Intron	3	0.5679	0.5679	0.5
JM35	(CT)12	F: CAGCGTCCCTCTCTCTCTTCC R: AGGAAGTTGAGGGACCAAATTGTA	Exon	2	0.6419	0.642	0.6
JM36	(AT)29	F: GAAAGCTAGAAATCAATGAACGCAC R: TCATTTAGTACATTGACCGGAGACA	Non genic	3	0.6419	0.642	0.6
JM37	(AAT)16	F: AATCACATCAGTTGTAACGGCA R: ATAATCTGATGGTTCAGTCAGCTCC	Non genic	3	0.7407	0.7407	0.7