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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Traffic. 2011 May 5;12(7):826–839. doi: 10.1111/j.1600-0854.2011.01202.x

FIG. 4.

FIG. 4

Cytoplasmic Vps75 NLS mutants are functional in vivo. (A) Whole cell lysates from RTT109-TAP and RTT109-TAP vps75Δ strains transformed with the indicated Vps75 plasmids were western blotted using antibodies against IgG (for TAP), HA, and PGK1 (loading control). KG, KG represents Vps75(K260G, K261G). (B) Ten-fold serial dilutions of the indicated strains transformed with Vps75 plasmids as shown were grown on CSM lacking uracil at 30°C (or 37°C where indicated) with 100mM HU or media lacking inositol as indicated. (C) Whole cell lysates from gcn5Δvps75Δ transformed with Vps75 plasmids as indicated were western blotted using antibodies against H3 K9ac and PGK1 (loading control).