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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Microsc. 2011 Feb 23;243(1):103–110. doi: 10.1111/j.1365-2818.2011.03490.x

Figure 2.

Figure 2

ROVAR uses image SBR to calculate starting and ending depth of each Z stack At each location in X-Y that is part of the imaging volume, the SBR is calculated at each depth. A threshold of SBR is set at which to locate the surface and the maximum imaging depth (A). Thus the image stack that is saved is only of high-SBR images at or above the threshold SBR (B).