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Viral antigen expression in Vero and ISE6 cells. Vero (left panel) or ISE6 (right panel) cells were infected with DEN4, LGT, LGT/DEN4, TBEV/DENΔ30, or TBEV/DENΔ30 mutant viruses at a multiplicity of infection of 1 or with medium (mock) and stained on day 4 (Vero) or 7 (ISE6) postinfection for the presence of virus antigen (green). 4′6-Diamidino-2-phenylindole (blue) was used to counterstain the nuclei.
