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. 2011 Jun;11(6):665–674. doi: 10.1089/vbz.2010.0179

FIG. 3.

FIG. 3.

Detection of viral RNA in ISE6 tick cells by RT-PCR. Total cellular RNA was isolated from cells infected with the indicated virus or from mock-inoculated cells. Viral RNA was detected using RT-PCR and primers specific for (A) positive- and (B) negative-sense strands of TBEV, LGT, or DEN4 virus RNA. Primers specific for TBEV were used to detect TBEV/DEN4Δ30 and its derivatives (vΔ30/E315, vΔ30/NS5654,655, and vΔ30/E315/NS5654,655). Primers specific to LGT or DEN4 virus genome were used to detect LGT, LGT/DEN4, or DEN4 RNA. Total RNA isolated from Vero cells infected with the corresponding virus was used as a positive control (+CNTRL) for detection of TBEV/DEN4Δ30, DEN4, LGT, or LGT/DEN4 RNA.