Table 2.

Infectivity of the Dengue 4, Langat, and Chimeric Viruses in Aedes aegypti Mosquitoes Fed on a Blood Meal Comprised of 10^5.5 PFU/mL of Virus

	No. infected/no. tested (percentage infected)b
	Day 14		Day 21
Virusa	Body	Head	Body	Head
DEN4	16/18 (89)c	13/16 (81)c	15/18 (83)c	15/15 (100)c
	3.5 ± 0.38d	2.8 ± 0.28d	3.7 ± 0.40d	3.0 ± 0.32d
TBEV/DEN4Δ30	0/18 (0)	0/18 (0)	0/18 (0)	0/18 (0)
vΔ30/E315	0/18 (0)	0/18 (0)	0/18 (0)	0/18 (0)
vΔ30/NS5654,655	0/18 (0)	0/18 (0)	0/18 (0)	0/18 (0)
vΔ30/E315/NS5654,655	0/18 (0)	0/18 (0)	0/18 (0)	0/18 (0)
LGT	0/18 (0)	0/18 (0)	0/18 (0)	0/18 (0)
LGT/DEN4	0/18 (0)	0/18 (0)	0/18 (0)	0/18 (0)

^aAedes aegypti mosquitoes were orally infected with the indicated viruses and incubated for 14 or 21 dpi. Mosquitoes ingested ∼2.8 log₁₀ PFU of virus, assuming that each mosquito ingested 2 μL of a 5.5 log₁₀ PFU/mL blood meal.

^bOn the indicated day, mosquito bodies and heads were harvested, triturated, and virus titer was determined on Vero cells.

^cIndicates that percent infected is statistically significant from remaining groups (p < 0.001; Fisher's exact test).

^dMean virus titer ± standard error (log₁₀ PFU/mosquito) of DEN4-infected mosquito bodies and heads are shown. These titers are significantly different from the remaining virus titers (p < 0.001; one-way analysis of variance, followed by Tukey post hoc test).