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Detection of Viral RNA in ISE6 Tick Cells During Three Serial Passages
| |
Passage 1a |
Passage 2 |
Passage 3 |
|||
|---|---|---|---|---|---|---|
| Virus | Positiveb | Negativeb | Positive | Negative | Positive | Negative |
| LGT | + | + | + | + | + | + |
| DEN4 | + | − | − | − | − | − |
| LGT/DEN4 | − | − | − | − | − | − |
| TBEV/DEN4Δ30 | + | + | − | − | − | − |
| vΔ30/E315 | + | + | − | − | − | − |
| vΔ30/NS5654,655 | + | + | − | − | − | − |
| vΔ30/E315/NS5654,655 | − | − | − | − | − | − |
Presence or absence of viral RNA is denoted by + or −, respectively.
Positive- or negative-sense RNA strand detected by RT-PCR.
