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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Cell Physiol. 2011 Nov;226(11):2881–2893. doi: 10.1002/jcp.22631

Fig. 10. The effects of decreasing NM II-A or II-B on LPA-induced migration in MDA-MB-231 cells.

Fig. 10

A. Measurement of LPA-induced migration after NMHC II-A or II-B siRNA treatment in MDA-MB-231 cells. Migration of NMHC II-A or II-B siRNA treated cells was measured in the presence or absence of LPA. These values represent the mean ± SE. B. LPA-mediated invasion of NMHC II-A, or II-B siRNA treated cells or control (luciferase) siRNA treated cells using matrigel-coated membranes. These values represent the mean ± SE. C. After transfection with indicated siRNAs for control (luciferase), NMHC II-A, and NMHC II-B, cells were treated with LPA for 10 min, harvested and then lysed. The supernatants were subjected to SDS 4–12 % PAGE. The membranes were probed with the indicated antibodies to NMHC II-A, II-B and actin. Actin was used as a loading control.