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. Author manuscript; available in PMC: 2013 Feb 10.
Published in final edited form as: Gene. 2010 Dec 30;493(2):195–200. doi: 10.1016/j.gene.2010.12.011

Figure 4. Role of the centromeric association of Pol III genes in the assembly of condensed mitotic chromosomes.

Figure 4

Immunofluorescent images of cells stained for tubulin (red) were merged with DAPI signals (blue; top panels). The c417 Pol III gene locus (green) and centromeres (magenta) visualized by FISH were merged with DAPI signals (blue; middle panels). Anaphase cells were subjected to the microscopic analysis. Intra-nuclear positioning of centromeres and the Pol III gene locus is depicted in the schematic diagrams (bottom panels). In wild-type cells (wt), centromeres associate with Pol III genes dispersed through the chromosomal arm regions and are pulled by spindle microtubules, and as a result chromosomes are properly segregated along the spindle microtubules during anaphase (left). In the cut3-477 condensin mutant, the centromeric association of Pol III genes is diminished and centromeres are segregated without holding the chromosomal arms (right). We hypothesize that tethering of Pol III genes to centromeres is a part of the proper assembly process for the condensed mitotic chromosomes, which is essential for faithful chromosome segregation.