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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nitric Oxide. 2011 Jan 26;25(2):59–69. doi: 10.1016/j.niox.2011.01.006

Figure 2.

Figure 2

A) Representative kinetic traces showing the consumption of the indicated deoxyhemoglobins (30μM) after the addition of sodium nitrite (750μM for bHb and THb, and 250μM for RHb) in PBS pH 7.4, 37°C and a fractional saturation of 0.45 – 0.5. B) Initial rate for the reaction between nitrite and deoxygenated bHb and THb as function of fractional saturation were calculated using the initial 10% of the deoxyhemoglobin consumption traces shown in panel A. The reaction was performed using 30μM Hb and a nitrite addition of 750μM. C) Initial rate for the reaction between nitrite and deoxygenated RHb as function of fractional saturation were calculated using the initial 10% of the deoxyhemoglobin consumption traces shown in panel A. The reaction was performed using 30μM Hb and a nitrite addition of 250μM.