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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nitric Oxide. 2011 Jan 26;25(2):59–69. doi: 10.1016/j.niox.2011.01.006

Table 1.

Biophysical properties of bHb, THb and RHb. The errors are standard deviation from triplicate reactions unless otherwise specified.

Solution P50 (mm Hg)a nb E1/2 (mV vs. SHE)c k′ox,NO (μM-1s-1)d MW (kDa)e
bHb 27.37±1.57 2.84±0.081 91.13± 11.51 (n=6) 18.3 ± 1.8 65.3±0.6
THb 41.16±3.05 1.01±0.015 65.53± 18.54 (n=12)* 18.7 ± 2.3 1330.3±159
RHb 1.84±0.78 0.69±0.11 ND 17.5 ± 5.5 6256±886.7
a

O2 affinity (i.e. pO2 at which the bHb/THb/RHb is half-saturated with O2),

b

cooperativity (Hill) coefficient,

c

reduction potential,

d

NO dioxygenation rate constant,

e

weight-average molar mass.

*

p<0.01 vs. bHb. ND – not determinable