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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Cell Biochem Funct. 2011 Jun;29(4):334–341. doi: 10.1002/cbf.1755

Figure 2.

Figure 2

ASB4 mRNA expression following hypoxia treatment. (A) Real time PCR analysis of ASB4 expression levels normalized to the first sample (+serum, 21% O2). MS1 cells were grown to confluency and treated for 16 hours with either serum containing medium or medium without serum. Afterwards, cells were grown for 24 hours under hypoxic or normoxic conditions. Results are shown as mean ± SD of three independent experiments. *p < 0.05, ***p < 0.001, ns = non-significant. (B) Real time PCR analysis of ASB4 expression levels. SVR cells were grown to confluency and treated with hypoxia for 0, 6 or 18 hours. Results are shown as mean ± SD of three independent experiments. Data are normalized to expression at 0 h; *p < 0.05.