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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Cell Biochem Funct. 2011 Jun;29(4):334–341. doi: 10.1002/cbf.1755

Figure 4.

Figure 4

ASB4 mRNA expression levels in MS1 cells following TNF-α or insulin treatment. Cells were serum starved for 16 hours before treatment. After treatment, real-time PCR analysis was performed. Data were normalized and significance calculated to expression at 0 hours. (A) MS1 cells were treated with 20 ng/ml TNF-α for 0, 8, 18 or 24 hours. (B) MS1 cells were treated with 0, 10, 20 or 40 ng/ml TNF-α for 24 hours. (C) MS1 cells were treated with 10 μg/ml insulin for 0, 18 or 24 hours. (D) MS1 cells (P. 13) were treated with 0, 1 or 5 μg/ml insulin for 18 hours. Results are shown as mean ± SD of three independent experiments. ***p < 0.0001, **p < 0.005, *p < 0.05, ns = non-significant.