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Effect of IKK-inhibitors on ASB4 mRNA expression levels. MS1 cells were treated with different concentrations of (A) BMS-345541 or (B) IKK Inhibitor VII one hour before TNF-α (20 ng/ml) was added for 24 hours. ASB4 expression levels were determined with real-time PCR and data normalized to ASB4 expression without stimulation by TNF-α. Results are shown as mean ± SD of three independent experiments. ***p < 0.001, ns = non-significant.
