Table 2.

Mating behavior of females injected with test substances and a control substance (Ringer's solution)

Test substances	Test		Control
	Mating	No mating	Mating	No mating
Oleic acid*	11	19	13	17
Linoleic acid†‡	3	27	22	8
Reduced plug§¶	12	18	14	16

Entries are number of queens with respective behavior. 

^*Pairwise posthoc test of oleic acid vs. control. Wald χ² = 0.277, df = 1, N = 30, P = 0.59. Univariate, χ² = 0.278; P = 0.6. 

^† Pairwise posthoc test of linoleic acid vs. control. Wald χ² = 19.039, df = 1, N = 30, P < 0.0001. Univariate, χ² = 24.754; P < 0.0001. 

^‡ For unknown reasons, controls mated unusually often in this experiment. However, the result is robust, because (i) assuming controls had mated only with a probability of 49.4% (the average for all controls in Tables 1 and 2), the test is: χ² = 11.429, P < 0.001, and (ii) assuming controls had mated with the lowest observed probability of 36.3% (as the controls in Table 1, “Sperm”), the test is χ² = 5.963, P = 0.02. 

^§ A mixture of oleic, palmitic, and stearic acids in the proportions as in natural plugs. 

^¶ Pairwise posthoc test of reduced plug vs. control. Wald χ² = 0.271, df = 1, N = 30, P = 0.60. Univariate, χ² = 0.271, P = 0.6.