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. Author manuscript; available in PMC: 2012 Jul 8.
Published in final edited form as: J Mol Biol. 2011 May 17;410(2):294–306. doi: 10.1016/j.jmb.2011.05.015

Figure 5.

Figure 5

Addition of hIAPP1–37 to 2.5 μM of the fluorescence zinc sensor, FluoZin-1, and 2.5 μM zinc. A decrease in fluorescence intensity corresponds to zinc being displaced from the zinc sensor and binding to the peptide. The solution was initially maintained at 4 °C to prevent aggregation. At the 6 μM titration point the temperature was raised to 25 °C. The peptide was allowed to stand at the 8 μM titration point until aggregation became evident by the turbidity of the solution. The increase in fluorescence intensity at 8 μM indicates the zinc is being displaced from the peptide by fibrillization. The decrease in fluorescence after addition of fresh peptide confirms the sensor is still binding zinc after fibrillization.