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. 2011 May 17;152(7):2904–2908. doi: 10.1210/en.2011-0230

Fig. 1.

Fig. 1.

Characterization of N-27 POR-G3H6. A, SDS-PAGE of purified N-27 POR-G3H6. B, Immunoblot of purified N-27 POR-G3H6. In A and B, lane M, molecular weight markers; lane 1, membrane protein; lane 2, flowthrough; lane 3, wash; lane 4, eluted fraction. C and D, Absorbance spectra. C, N-27 POR-G3H6. D, N-27 POR. E and F, Lineweaver-Burk analyses; circles represent the activity of N-27 POR and triangles represent the activity of N-27 POR-G3H6. E, 17α-Hydroxylase activity. F, 17,20-Lyase activity. Error bars represent the mean ± sem of at least three independent experiments.