Table 1.
Catalytic activities of human P450c17 supported by N-27 POR and N-27 POR-G3H6
| 17α-Hydroxylase |
17,20 Lyase |
|||
|---|---|---|---|---|
| N-27 POR | N-27 POR-G3H6 | N-27 POR | N-27 POR-G3H6 | |
| Km (μm) | 1.73 ± 0.154 | 1.49 ± 0.344 | 1.92 ± 0.095 | 1.89 ± 0.341 |
| Vmax (pmol steroid/pmol P450/min) | 0.029 ± 0.0007 | 0.026 ± 0.004 | 0.041 ± 0.002 | 0.042 ± 0.005 |