Fig. 3.

Impaired masculine characteristics in the male Gli2 mutant GT. A, The fold expression of MafB and Fkbp5 between male and female GT is decreased in the Gli2 mutant GT. Gli1 expression does not show sexually dimorphic expression pattern. RNA was isolated from the mesenchyme adjacent to the UPE (blue area in A). The relative RNA equivalents for each sample were determined by standardization with ribosomal protein L8 levels. Statistical significance is indicated by asterisks, with P < 0.05. B, The expression of Dkk2 and Sfrp1 is up-regulated in the Gli2 mutant GT in comparison with those of the wild-type (WT) male GT at E15.5. Their expression pattern is shown by the representative transverse sections.