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. 2011 Feb 1;149(5):551–562. doi: 10.1093/jb/mvr014

Table I.

Activity of rSynPTP towards exogenous substrates.

Specific activity
Recombinant protein RCML (32P-Tyr) (pmol/min/mg) Casein (32P-Tyr) (pmol/min/mg) pNPP (µmol/min/mg)
SynPTP 800 ± 20 1380 ± 70 3.1 ± 0.16
Cys7Ser-SynPTP ND ND ND

The catalytic activity of rSynPTP or mutagenically altered SynPTP was determined using 500 ng of each recombinant protein and 4 µM Casein (32P-Tyr)/RCML (32P-Tyr) or 10 mM pNPP as exogenous substrates as described in the ‘Materials and Methods’ section. ND, not detected, ≤2%.