Table I.
Specific activity |
|||
---|---|---|---|
Recombinant protein | RCML (32P-Tyr) (pmol/min/mg) | Casein (32P-Tyr) (pmol/min/mg) | pNPP (µmol/min/mg) |
SynPTP | 800 ± 20 | 1380 ± 70 | 3.1 ± 0.16 |
Cys7Ser-SynPTP | ND | ND | ND |
The catalytic activity of rSynPTP or mutagenically altered SynPTP was determined using 500 ng of each recombinant protein and 4 µM Casein (32P-Tyr)/RCML (32P-Tyr) or 10 mM pNPP as exogenous substrates as described in the ‘Materials and Methods’ section. ND, not detected, ≤2%.