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TOAC-labeled PLB in lipid bilayer: probability distribution of PLB domains orientation in a membrane, revealed from 9.4 and 94 GHz EPR spectra. TOAC label is rigidly attached to PLB backbone and distribution of spin probe’s z-axis orientation, measured by EPR reflects distribution of PLB domain orientations. a Transmembrane domain, very narrow distribution; b cytoplasmic domain, T conformation; c cytoplasmic domain, R conformation, practically unrestricted motion
