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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: J Neuroparasitology. 2010 Dec;1:N101001. doi: 10.4303/jnp/N101001

Figure 3.

Figure 3

Reactivity of anti-BPVM with T. gondii bradyzoite vacuoles in vitro. Panel A: phase contrast microscopy (40× objective) of T. gondii in a human fibroblast cell demonstrating a refractile vacuole membrane. The bar is 15 μm. Panel B: reactivity of this vacuole (arrow) with polyclonal anti-BPVM mouse antiserum detected with fluorescein antimouse IgG demonstrating staining of the vacuole membrane. Panel C: reactivity of the vacuole with anti-BAG1 rabbit antibody detected with Texas red antirabbit IgG demonstrating that this vacuole contains bradyzoites. Panel D is an overlay of Panels B and C demonstrating the reactivity of anti-BPVM with the vacuole membrane.