Table 1. Primers used for CRYBA1 amplification.
Exon | Primer (5′-3′) | Product length (bp) | Annealing temperature (°C) |
---|---|---|---|
CRYBA1-1F |
GGCAGAGGGAGAGCAGAGTG |
|
|
CRYBA1-1R |
CACTAGGCAGGAGAACTGGG |
207 |
52 |
CRYBA1-2F |
AGTGAGCAGCAGAGCCAGAA |
|
|
CRYBA1-2R |
GGTCAGTCACTGCCTTATGG |
293 |
52 |
CRYBA1-3F |
AAGCACAGAGTCAGACTGAAGT |
|
|
CRYBA1-3R |
CCCCTGTCTGAAGGGACCTG |
269 |
52 |
CRYBA1-4F |
GTACAGCTCTACTGGGATTG |
|
|
CRYBA1-4R |
ACTGATGATAAATAGCATGAACT |
357 |
52 |
CRYBA1-5F |
GAATGATAGCCATAGCACTAG |
|
|
CRYBA1-5R |
TACCGATACGTATGAAATCTGA |
290 |
52 |
CRYBA1-6F |
CATCTCATACCATTGTGTTGAG |
|
|
CRYBA1-6R | GCAAGGTCTCATGCTTGAGG | 295 | 52 |
Cited from [6]