Figure 1.

Abdominal (epidydimal) and subcutaneous (s.c.) fat pads as well as spleen, LN, lung and liver were isolated from retired-breeder B6 male mice, and the SVF fraction was stained for Foxp3, CD3, CD4, CD8 and CD25. (a) Upper panel: T cell distribution in SVF fraction from abdominal fat tissue. Numbers on top indicate the mean and standard deviation (SD) for cells in the lymphocyte gate after fixing and permeabilization, fraction of CD3⁺ T cells among lymphocyte-gated cells and distribution of CD4⁺ and CD8⁺ T cells. Lower panel: Percentage of Foxp3⁺CD25⁺ T cells in abdominal fat tissue gated on CD4⁺ or CD8⁺ T cells. Organs of 5 mice were pooled. Representative dot plots are shown. (b) Frequency of Foxp3⁺CD4⁺ T cells in different organs. Mean and SD from at least three independent experiments are shown, whereas organs from 4–5 mice per experiment were pooled. (c) Kinetic of T_reg cell appearance in abdominal and s.c. fat tissue as well as in spleen. (d) Immunohistology of abdominal adipose tissue. Arrow-head indicates Foxp3 staining. Note: Foxp3 expression is restricted to the nucleus. * refers to dead-adipocyte residue surrounded by a crown-like structure formed by immune cells. Control staining with isotype antibody. Original magnification: left panel, upper picture 400x, all other 1000x.