Figure 4.

Three mouse models of obesity: Lep^ob/ob, A^y/a and HFD. (a–c) Abdominal adipose tissue from Lep^ob/ob and heterozygote Lep^ob/wt mice was analyzed for T_reg cell frequency. (a) Representative dot plots of 13-week-old mice. (b) Total number of T_reg cells per one gram fat. (c) Changes of T_reg representation over age. Mean and SD are shown. (d) Percentage of T_reg cells in abdominal adipose tissue of 24-week-old A^y/a or littermate (WT) mice. (e) Percentage of fat T_reg cells in mice fed for 29 weeks with HFD or NC. (f) Correlation of HOMAR-IR and fraction of T_reg cells. (g–i) Observed changes of T_reg cell proportion in adipose tissue of the three obesity models were not reflected in other organs. (g) Lep^ob/ob, (h) A^y/a, (i) HFD.