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GrTP induced recruitment of FADD to Fas/CD95. IEC-6 cells were treated with 400µg/ml of GTP for the indicated time. The Fas/CD95 and its associated proteins were immuno-precipetated using anti-Fas antibody. The presence of FADD was identified by Western immunoblotting. Equal loading of samples was verified by reprobing the membrane with anti-Fas antibody.
