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. 2011 Apr 15;124(8):1301–1307. doi: 10.1242/jcs.084277

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© 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 4. — WRBcc interacts with TRC40. (A) Purified GST–TRC40 and MBP–WRBcc interact with each other. MBP (lane 1) and MBP–WRBcc (lane 3) were bound to amylose resin. GST–TRC40 (lane 2) was then incubated with the resins, and the unbound and bound proteins characterized by SDS-PAGE and Coomassie Blue staining. (B) HisWRBcc co-immunoprecipitates with TRC40 and TRC40 with HisWRBcc. HisWRBcc (lanes 1–4) and TRC40 (lanes 9–12) were expressed alone or together (lanes 5–8 and 13–16) in HeLa cells, and either TRC40 (left panel) or hisWRBcc (right panel) was immunoprecipitated by their respective antibodies (anti-TRC40 or anti-his). Co-immunoprecipitated proteins were then characterized by western blot analysis using the anti-his (left panel) or anti-TRC40 (right panel) antibody.