Figure 5.
Deletion of CBP6 provokes a phenotype similar to that of Δcbp3 cells. (A) Cytochrome b is destabilized in the absence of Cbp6. Mitochondrial translation products from wild-type or Δcbp6 cells were analyzed as described in Fig. 2 (C and D). (B) Cbp6 is critical for efficient translation of a cob::ARG8m reporter. Cells of wild type, Δcbp3, or Δcbp6 harboring the modified mitochondrial genome described in Fig. 3 A were plated in serial 10-fold dilutions onto synthetic media (SD) containing or lacking arginine and incubated for 2 d. (C) The absence of Cbp6 impairs translation of COB-ARG8m-COB mRNA. Mitochondrial translation products of wild-type and Δcbp6 cob::ARG8m cells were labeled with [35S]methionine and analyzed by autoradiography. MM, molecular mass.