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. 2011 Jun 13;193(6):1049–1064. doi: 10.1083/jcb.201012035

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© 2011 Ricke et al.

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Figure 4. — Aurora B activity is increased in Bub1-overexpressing cells. (A) Representative images of wild-type and Bub1^T264 prophase cells immunostained for pCenp-A and centromeres. DNA was visualized with Hoechst. Bar, 10 µm. (B) Quantification of the pCenp-A signal using ImageJ software. Error bars represent SEM. *, P < 0.05 vs. wild type (unpaired t test). (C) Representative images of wild-type and Bub1^T264 prophase cells immunostained for pKnl1 and centromeres. DNA was visualized with Hoechst. Bar, 10 µm. (D) Quantification of the pKnl1 signal using ImageJ software. Error bars represent SEM. *, P < 0.05 vs. wild type (unpaired t test). (E) Mitotic extracts of wild-type and Bub1^T264 cells subjected to immunoprecipitation with Bub1, Aurora B, or IgG antibodies and analyzed by Western blotting as indicated. (F) Mitotic extracts prepared from taxol-treated HeLa cells subjected to immunoprecipitation with Bub1, Aurora B, or IgG antibodies and analyzed by Western blotting as noted.