Table I.
Overexpression of Bub1 in MEFs induces progressive near-diploid aneuploidy
| MEF genotype (n) | Mitotic figures inspected | Aneuploid figures (SD) | Karyotypes with indicated chromosome number | Mitotic figures with PMSCS (SD) | ||||||
| 37 | 38 | 39 | 40 | 41 | 42 | 43 | ||||
| % | % | |||||||||
| WT (3) | 150 | 11 (1) | 2 | 1 | 5 | 133 | 7 | 2 | 1 (1) | |
| Bub1T85 (3) | 150 | 21 (1) | 2 | 1 | 9 | 118 | 15 | 5 | 8 (0) | |
| WT (3) | 150 | 11 (1) | 5 | 133 | 8 | 3 | 1 | 3 (1) | ||
| Bub1T264 (3) | 150 | 25 (1) | 1 | 3 | 22 | 113 | 9 | 2 | 12 (1) | |
Empty spaces mean that there were no karyotypes with the indicated chromosome number. Karyotyping was performed at passage 5.