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. 2001 Mar 27;98(7):3940–3945. doi: 10.1073/pnas.061026198

Figure 2.

Figure 2

Visualization of ODN probes hybridized to 50-mer target sequences in the CFTR gene in interphase nuclei and DNA fibers. (A) RCA detection of probes targeted to the G542X locus (FITC), the Δ508 locus (Cy-3), and the M1101K locus (Cy5) in normal human lymphocytes. Merged images (com) show that signals from all three loci colocalize. (B) Hybridization of the Δ508 locus probe to nuclei of HeLa cells. (C) Cohybridization of two PAC clones (extended green and red signals) with Δ508 (yellow), G542X (green), and M1101K (white) oligomer probes. Eight patterns (a–h) of hybridization are produced (see text).