Table 1.

Physical mapping of three loci in the CFTR gene region by RCA

Locus	ODN sequences
Δ508	Probe-primer PRP3 (89): 3′-CCCTCTTGACCTCGGAAGTCTCCCATTTTAATTCGTGTCACCTTCTTAAAtttt(CH2)18tttttACGTCATCATGAACATTACACGTTCCAC-3′
	Circle3 (78): GTGGAACGTGTAATGTTCATGATGACGTGCATCCTTGACAGCCGATGAGGCTGGCATCCTTGACAGCCGATGAGGCTG
	Decorator probe: Det3-Cy3 (24): 5′-Cy3-GCATCCTTGACAGCCGATGAGGCT-3′
G542X	Probe-primer PRP1 (89): 3′-GAACCTCTTCCACCTTAGTGTGACTCACCTCCAGTTGCTCGTTCTTAAAGtttt(CH2)18tttttATGATCACAGCTGAGGATAGGACATGCGA-3′
	Circle1 (78): CGCATGTCCTATCCTCAGCTGTGATCATCAGAACTCACCTGTTAGACGCCACCAGCTCCAACTGTGAAGATCGCTTAT
	Decorator probe: Det1c-FITC (18): 5′-FITC-TCAGAACTCACCTGTTAG-3′; Det1d-FITC (18): 5′-FITC-ACTGTGAAGATCGCTTAT-3′
M1101K	Probe-primer PRP4 (89): 3′-GACGGTTGACCAAGAACATGGACAGTTGTGACGCGACCAAGGTTTACTCTtttt(CH2)18tttttCTTGTACATGTCTCAGTAGCTCGTCAGT-3′
	Circle4 (78): ACTGACGAGCTACTGAGACATGTACAAGGAGCAGTCCTGṪCAGCTAGGTCACGGAGCAGTCCTGTCAGCTAGGTCACG
	Decorator probe: Det4-Cy5 (24): 5′-Cy5-GAGCAGTCCTGTCAGCTAGGTCACG-3′

Bold type: probe sequence; lowercase tttt(CH₂)₁₈ttttt: linker; standard type: RCA primer, circle, and decorator ODN sequences. Note that probe-primer and AD-P2-ODNs have polarity reversal and two 3′ ends.