Table 2.

Sequences for discrimination of mutations by RCA

Gene	Anchor probes (P1) and AD (P2) probes
CFTR	P1 5′-AACGAGCAACTGGAGGTGAGTCACACTAAGGTGGAAGAG-3′; P2Wt 3′-GTTCTTGATATAACAGAAAGtttt(CH2)18tttttATGATCACAGCTGAGG- ATAGGACATGCGA-3′; P2Mu 3′-TTTCTTGATATAACAGAAAGtttt(CH2)18tttttACGTCGTCCGTGCTAGAAGGAAACACGCA-3′
P53	P1 5′-GTTGAGGGCAGGGGAGTACTGTAGGAAGAGGAAGGAGACA-3′; P2Wt 3′-TTCTACAAAACGGTTGACCtttt(CH2)18tttttATGATCACAGCTGAGG- ATAGGACATGCGA-3′; P2Mu 3′-GTCTACAAAACGGTTGACtttt(CH2)18tttttACGTCGTCCGTGCTAGAAGGAAACACGCA-3′
Patched	P1 5′-ACACGGCGCCATCCAGGACGGGTGCAAACATGTGCTCCAG-3′; P2Wt 3′-GGTGAGACGACCCTCtttt(CH2)18tttttATGATCACAGCTGAGGATA- GGACATGCGA-3′; P2Mu 3′-TGTGAGACGACCCTCtttt(CH2)18tttttACGTCGTCCGTGCTAGAAGGAAACACGCA-3′
BRCA1	P1 5′-ATTCTCTTGCTCGCTTTGGACCTTGGTGGTTTCTTCCATT-3′; P2Wt 3′-GGGTCCTGTCTTTCTAGAAGtttt(CH2)18tttttATGATCACAGCTGAGGAT- AGGACATGCGA-3′; P2Mu 3′-GGGGTCCTGTCTTTCTAGAAtttt(CH2)18tttttACGTCGTCCGTGCTAGAAGGAAACACGCA-3′
Circle sequences:  Wt: CGCATGTCCTATCCTCAGCTGTGATCATCAGAACTCACCTGTTAGACGCCACCAGCTCCAACTGTGAAGATCGCTTAT Mu: GCGTGTTTCCTTCTAGCACGGACGACGTATATGATGGTACCGCAGCCAGCATCACCAGACTGAGTATCTCCTATCACT
Decorator probe sequences:   Wt: Det1a-Cy5: 5′-Cy5-TCAGAACTCACCTGTTAGACGCCA; Det1b-Cy5: 5′-Cy5-GCTCCAACTGTGAAGATCGCTTAT  Mu: Det2a-Cy3: 5′-Cy3-TATATGATGGTACCGCAGCCAGCA; Det2b-Cy3: 5′-Cy3-CCAGACTGAGTATCTCCTATCACT

Bold type: probe sequence; lowercase tttt(CH₂)₁₈ttttt: linker; standard type: RCA primer, circle, and decorator ODN sequences. Note that probe-primer and AD-P2-ODNs have polarity reversal and two 3′ ends.