Figure 3. Retigabine and bradykinin affect the chronotropic response of the cardiomyocytes to nicotine stimulation of the co-cultured SCG neurons.

A, CR is plotted during an experiment as nicotine (1 μm), nicotine (1 μm) + retigabine (10 μm), bradykinin (250 nm), Isopr (10 μm), or Isopr + Propr (both 10 μm) were bath-applied to co-cultured rat SCG and B₂ k/o mouse cardiomyocytes. B, summarized experiments as in A, in co-cultured rat SCG and wt mouse cardiomyocytes, showing the inhibitory effect of retigabine. C, summarized experiments as in A, in co-cultured rat SCG and B₂ k/o mouse cardiomyocytes, showing the potentiating effect of bradykinin on the response to nicotine. In B and C, each point summarizes 4–5 measurements, with the SEM for each point shown.