Figure 1.

Representative examples of genes/ORFs whose overexpression causes >5->20% shift toward 1C (A) or 2C (B). DNA content. Cells carrying control plasmid (pRS316-GAL1) or plasmids encoding genes/ORFs of interest were induced in galactose containing medium for 6–8 h, and DNA content was assayed by flow cytometry. Photographs of induced cultures are included to demonstrate presence or absence of a homogeneous terminal morphology.