Figure 2.

Aberrant promoter hypermethylation is rare in the MAP2K4 promoter region. A. Map of MAP2K4 promoter region from UCSC Genome browser (Mar. 2006 build) showing the gene in blue (exon 1 indicated by thicker bar), predicted CpG island in green, location of all CpG dinucleotides in orange, and location of the primers and MS-SSCP PCR product in black. MS-SSCP (B) and DNA sequencing electrophoretograms (C) for a representative tumor sample lacking methylation (top) and the positive methylation control SssI treated normal lymphoblast DNA.