Figure 2.

Optimization of Agrobacterium infection pre-treatments for T-DNA transfer into safflower cotyledon explants. (A) Effect of bacterial density on transient expression of GFP in cotyledons of S-317 and WT. (B) Acetosyringone and spermidine concentration in inoculation media (Winnans AB salts) and its effect on GFP expression two weeks after Agrobacterium co-cultivation (OD600 = 0.4). Each value is the mean ± SE of three independent experiments, each with 30 cotyledons. Significant differences between treatments are indicated by different letters.