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. 2011 Jun 15;6(6):e19811. doi: 10.1371/journal.pone.0019811

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Peripheral lymphocytes were analyzed for CD4-FITC, CD25-PE and Foxp3-APC expressions. The graphs showed that CD4⁺ T cells gated by R2 were isolated from lymphocytes and divided into CD4⁺CD25⁺(R3), CD4⁺CD25⁺Foxp3⁺ (R4), CD4⁺CD25^low (R5) and CD4⁺CD25^high (R6). Data represented the results of one representative experiment out of seven performed under the same conditions.