Figure 5. Changes of TLR4 expression on CD4⁺CD25⁺Tregs.

CD4⁺CD25⁺Tregs (R2) and CD4⁺CD25⁻T cells (R1) were isolated from the peripheral blood of male BALB/c mice by magnetic beads and analyzed by flow cytometry. The expression of TLR4 on CD4⁺CD25⁺Tregs was stained with the indicated FITC-antibodies. Data were represented by a histogram in which gray area represents isotype-matched control mAbs, solid lines represent CD4⁺CD25⁺Tregs isolated from sham burn mice, and dotted lines represent CD4⁺CD25⁺Tregs also stimulated with APS(200 µg/ml) for 24 hr or isolated from burn plus infection mice at PBD7. Data represent the results of one representative experiment out of seven.