Table 1. Pharmacology and specificity of GmPT1 and GmPT2.
Inhibitor | 32P uptake (% of the control) | |
GmPT1Mean ± SE | GmPT2Mean ± SE | |
CCCP (10 µM) | 93±0.04 | 96±0.06 |
CCCP (100 µM) | 77±0.02 | 80±0.05 |
DNP (10 µM) | 92±0.02 | 94±0.07 |
DNP (100 µM) | 79±0.05 | 82±0.01 |
Vanadate (10 µM) | 92±0.02 | 96±0.05 |
Vanadate (100 µM) | 82±0.03 | 83±0.04 |
NH4Cl (5 mM) | 78±0.08 | 76±0.05 |
KCl (5 mM) | 75±0.03 | 72±0.07 |
NaAc (5 mM) | 77±0.08 | 78±0.04 |
CCCP, carbonyl cyanide m-chlorophenylhydrazone.
DNP, 2,4-dinitrophenol.
Inhibitors were added to yeast cells 30 s before addition of labeled Pi. All assays were done at pH 4. Values for each treatment were derived from three independent measurements. Water was used as the control treatment.