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. 2011 Jun 15;6(6):e19752. doi: 10.1371/journal.pone.0019752

Table 1. Pharmacology and specificity of GmPT1 and GmPT2.

Inhibitor 32P uptake (% of the control)
GmPT1Mean ± SE GmPT2Mean ± SE
CCCP (10 µM) 93±0.04 96±0.06
CCCP (100 µM) 77±0.02 80±0.05
DNP (10 µM) 92±0.02 94±0.07
DNP (100 µM) 79±0.05 82±0.01
Vanadate (10 µM) 92±0.02 96±0.05
Vanadate (100 µM) 82±0.03 83±0.04
NH4Cl (5 mM) 78±0.08 76±0.05
KCl (5 mM) 75±0.03 72±0.07
NaAc (5 mM) 77±0.08 78±0.04

CCCP, carbonyl cyanide m-chlorophenylhydrazone.

DNP, 2,4-dinitrophenol.

Inhibitors were added to yeast cells 30 s before addition of labeled Pi. All assays were done at pH 4. Values for each treatment were derived from three independent measurements. Water was used as the control treatment.