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. 2011 Jun 15;6(6):e20866. doi: 10.1371/journal.pone.0020866

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Zhuang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Co-staining of adult testis sections for the DAPI(blue)and SLX (green) using anti-SLX^95–212aa; (B) Immunostaining of adult testis sections using anti-SLXL1^1–155aa; (C–D) A Stage VIII tubule immunostained by antisera for SLXL1 (red) and γH2AX (green); (E–F) A Stage VI-VII tubule showing the SLXL1 (red) localization in the round spermatid and elongating spermatozoa. (G) Immunostaining of SLXL1 in the spermatozoa of epididymis (red) showing its localization in the acrosome. (H) SLXL1 (red) was present in the acrosome of mature spermatozoa. D and F are the magnified views of the rectangular areas in C and E, respectively.