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. 2011 Jun 15;6(6):e21018. doi: 10.1371/journal.pone.0021018

Figure 5. Affinity measurements by surface plasmon resonance.

Figure 5

Shown are Biacore ×100 sensorgrams obtained for the binding of Fab and IgG1 R11, R12, and Y31 to immobilized Fc-hROR1 (A) and Fc-mROR1 (B) after instantaneous background depletion. The mAbs were injected at five or six different concentrations (shown in different colors) ranging from 1.5 to 100 nM. Each concentration was tested in duplicates.