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. 2011 Jun 15;6(6):e21018. doi: 10.1371/journal.pone.0021018

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Flow cytometry was used to identify PBMC subpopulations from patients CLL-2 (A), CLL-3 (B), CLL-4 (C), and CLL-5 (D) as NK cells (CD16+ CD3−), T cells (CD16− CD3+ and CD19− CD5+), and CLL cells (CD19+ CD5+). The binding of biotinylated IgG1 R12 (blue; 1 µg/mL) to total PBMC is shown at the bottom. The gray shade indicates the background observed with PE-streptavidin alone. The x and y axes in the top and middle row and the x axis in the bottom row depict the fluorescence intensity, the y axis in the bottom row the number of events.