Table 1. Panel of chimeric rabbit/human Fab selected by phage display.
Clone1 | Library | Panning rounds | Repeated clones | Binding | |||
hROR1ECD | Fc-hROR1 | hROR1ECD2 | Fc-hROR12 | HEK 293F/hROR13 | |||
R11 | R | 4 | 0 | 26/31 | ++ | ++ | + |
R12 | R | 4 | 0 | 1/31 | ++ | ++ | ++ |
Y4 | Y | 4 | 0 | 2/31 | ++ | − | − |
Y13 | Y | 4 | 0 | 14/31 | ++ | − | − |
Y14 | Y | 4 | 0 | 2/31 | + | − | − |
Y27 | Y | 4 | 0 | 13/31 | ++ | − | − |
Y31 | Y | 3 | 1 | 4/4 | + | + | + |
Defined by unique DNA fingerprint and sequence.
As measured by ELISA.
As measured by flow cytometry.